摘要
以[^(32)P]-dATP标记含AcNPV DNA的EcoRI-I片段的重组质粒为探针,在35℃条件下对油桐尺蠖核多角体病毒(BsNPV)多角体蛋白基因进行了定位,将其分别定位在BamH Ⅰ-A,Bgl Ⅰ-A,Bgl Ⅱ-F,EocR Ⅰ-R,Hind Ⅲ-A,Kpn Ⅰ-Ⅰ,Pst Ⅰ-D,Xba Ⅰ-A(或B),Xho Ⅰ-F和G片段上,并以M13mp18为载体,克隆了Kpn Ⅰ-Ⅰ片段。
The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA EcoRI-I as the probe. For sequencing the polyhedringene, the KpnI-I fragment was cloned into M13mp18 vector.
出处
《中国病毒学》
CSCD
1992年第1期80-84,共5页
Virologica Sinica
基金
国家自然科学基金资助课题
关键词
油桐尺蠖
核多角体病毒
Buzura suppressaria nuclear polyhedrosis virus(BsNPV)
Polyhedrin gene Hybridization
