摘要
用间接ELISA、ELISA交叉阻断法和交叉血凝抑制试验对兔出血症病毒(RHDV)与6种细小病毒进行抗原相关性试验。用间接ELISA证实,RFIDV与它们有轻度交叉关系,其抗原相关值分别为:小鼠细小病毒(MVM)5.59%;鹅细小病毒(GPV)3.54%;猪细小病毒(PPV)1.76%;水貂肠炎病毒0.7%。细小病毒间的抗原相关值:MEV与PPV为31.6%,MEV与MVM为35.36%;而CPV与MEM、PPV、MVM的相关值均为零,即无相关性。在ELISA交叉阻断法中证实:犬细小病毒(CPV)、猫泛白细胞减少症病毒(FPV)和MEV均不能阻断RHDV与其抗体结合,仅GPV有轻度阻断作用,其最大阻断率为40%。在血凝交叉抑制试验中,未发现RHDV与细小病毒及其相应抗体间存在交叉抑制现象。以上结果表明RHDV与细小病毒在血清学方面有轻度相关性。
Antigenic relationship between rabbit hemorrhagic disease virus (RHDV) and six parvoviruses was determined with indirect enzyme-linked immunosorbent assay (indirect-ELISA), enzyme-linked immunosorbent inhibition assay (ELISIA) and hemagglutination inhibition (HI) test. The results by indirectELISA showed that the correlation coefficients between RHDV and minute virus of mice (MVM), goose parvovirus (GPV), porcine parvovirus (PPV) and mink enteritis virus (MEV) were 5.59%, 3.54%, 1.76% and 0.7%, respectively, while the correlation coefficients between MEV and PPV was 31.6% and between MVM and MEV was 35.3%. There was no relationship between GPV and other parvoviruses including MVM, MEV and PPV. In ELISA inhibition test relationship did not exist between RHDV and feline parvoviruses (including CPV, MEV and FPV) except GPV whose highest inhibition rate was 40%. There was no evidence of any relationship between RHDV and parvoviruses by HI test. Based on cross reaction, RHDV was correlated with parvoviruses to some extent.
出处
《中国病毒学》
CSCD
1992年第3期351-356,共6页
Virologica Sinica
关键词
兔出血症病毒
细小病毒
抗原相关性
Rabbit hemorrhagic disease virus Parvovirus Indirect-ELISA HI test