摘要
本工艺的特点是以人工饲料饲养油桐尺蠖幼虫,用昆虫细胞系增殖病毒作为毒源,感染4龄幼虫,收集病死虫,提取多角体,加工制成杀虫剂,产品经安全性检测,并进行田间试验,有较好的杀虫效果。
The oil tree moth (B. suppressaria larvae) was grown to 4 instar using a seml-synthetic artificial diet. then infected with (BsNPV) inoculum propagated in the insect cell cultures. 7 days p.i., the virus-infected larvae were collected and virus (PIB) was purified by differential centrifugation. Then, a flowable formulation was made with the glycerin, virus-protective agent, emulsion and antibiotic preparation was determined by standard bioassays using laboratory-reared larvae on artificial diet. LC50 was 2.36×10~3 PIB/ml. The safety test was done and the field trial was fully effective.
出处
《中国病毒学》
CSCD
1992年第3期304-310,共7页
Virologica Sinica
关键词
病毒杀虫剂
油桐尺蠖
核多角体病毒
Artificial diet for insect Cell culture Viral insecticide