酶联免疫吸附法(ELISA)检测四川“榨菜”病毒种类

Kinds of Virus Causing Viral Disease on Sichuan Zha-Cai (Brassica juncaa var. tumida) as Detected by ELISA Method

1990至1991年在“榨菜”病毒病发生的始期和盛期,从四川九个县(市)近郊、远郊采集样品492份,用TuMV、CMV、TMV、PVX、PVY和CaMV六种抗血清,经酶联免疫吸附间接法进行检测,属于TuMV和TuMV与其它五种病毒之一或之二复合感染的有422份,占总样品数的85.77％,其中由TuMV单一感染的305份,占61.99％。此外,还有PVX、PVY、CaMV单一感染病样27份,占5.49％,而情况不明的43份,占8.74％,总检出率91.26％。检测结果表明,发病始期或盛期,近郊或远郊均以TuMV和TuMV与CMV复合感染的病毒为主,是四川“榨菜”生产上主要的病毒种类。

In 1990—1991. from the fields in near- and-far suburb of nine counties or municipalities in Siehuan province, 492 accessions of Brassica juncea plant showing viral disease symptom were collected both in the initial and the prevalent period of the disease and were subjected to indirect ELISA tests with 6 kinds of antiserum—— TuMV, CMV. TMV. PVX. PVY. CaMV—— in an attempt to find their causal agent. 422 speeimens, accounting for85.77％, of the total, showed reaction with TuMV antiserum or with antiserum of one or two of the rest five viruses in addition to that of TuMV. 305 (61.99％ of the total) of these 422 specimens could be comfirmed as TuMV mono-infected. Furthermore, of the rest speeimens, 27 (5.49％ of the total) were confirmed to be mono-infeeted by PVX, or PVY. or CaMV. while 43(8.74％ of the total) were unclarified yet by the present test, Thus. 91.26％ of these 492 specimens were identified for their causal viral agent. The results suggest that TuMV or TuMV+CMV complex are the predominant causal viruses infecting Brassiea juncea both in the initial and the prevalent periods of the disease in either near-or-far suburban fields, According to the present results of ELISA test, they are the major viruses imposing heavy damage on Sichuan Zha-Cai in its growing areas.