油桐尺蠖核型多角体病毒在Bs484细胞中的感染特性研究

Study on the Infective Characters of Buzura suppressaria Nuclear Polyhedrosis Virus in BS484 Cell Line

本文研究了油桐尺蠖核型多角体病毒(简称BsNPV)在油桐尺蠖成虫卵巢细胞系(Bs484)中的以下感染特性:1.病毒接种传代3-4天的细胞时,病毒感染率最高;2.病毒按种量在一定范围内与感染细胞的多角体总产量平行;3.病毒在细胞中连续传代七次后其滴度无明显变化;4,病毒基因组在感染细胞后6小时左右开始合成,并于感染后14小时达到最大。此外,本实验还发展了一种用于检测感染细胞中的病毒核酸的简便方法。

This thesis reported the following infective charaeters of Buzura suppressatia nuclear polyhedrosis virus (BsNPV) in Bs484 cell line: 1. The viral infection rate was the highest when the virus was inoculated in Bs484 cells had been subeultured for 3—4 days. 2. The viral inocuiating quantities paralleled the total yield of polyhadra in virus-infected cells in certain range. 3. The viral tite had (?)o significant change after BsNPV was serially passaged for sevan times in Bs484 cells. 4. The synthesis of the viral genome began about 6 hours postinfection in BsNPV-infected cells and reached the maximum around 14 hours postinfection. In addition, this experiment also developed a handy method fot checking viral nuclcic acids in virus-infeeted cells.