水稻条纹叶枯病毒分子生物学的研究——Ⅲ.外壳蛋白基因的序列分析

Study on Molecular Biology of Rice Stripe Virus Ⅲ. Sequence Analysis of Coat Protein Gene

以含有水稻条纹叶枯病毒(RSV)外壳蛋白(CP)基因的重组克隆为材料,将RSV-cDNA酶解后,亚克隆人M13mp18、19,采用双脱氧链终止法测序得到RSV-CP基因的全长序列(共含966bp),同日本已发表的RSV-CP基因序列相比同源率达97％。

We used the recombinant clone eontaing RSV-CP gene as material. After being digested with restriction endonueleases, RSV-eDNA fragments were subcloned into M13 rap18, 19. The CP gene sequence was determined by dideoxynucleotide chain termination method and the result revealed that RSVCP gene consists of 966 base pairs with 97% sequence homology to the Japanese isolate.