在培养的心肌细胞中锌的抗氧自由基损伤作用的观察

Observation of effects of zinc on the injury induced by oxygen free radical in cultured heart cells

本文通过向培养基中加入黄嘌呤和黄(?)呤氧化酶系统成分造成培养的心肌细胞受氧自由基伤害,以电生理的改变和BaCL_2引起心肌细胞停搏的闭浓度为指标,观察了微量元素Zn的抗氧化损伤作用。结果示:XOD组与对照组比较,动作电位APA、OS、MDP、TP、Vmax及APD_(50)各电参数明显减小。BaCl_2引起心肌细胞停搏的阈浓度亦减低。而加Zn组与对照组比较,以上结果除TP值偏低P<0.05外,其它参数则无显著性改变。加Zn组与XOD组相比,动作电位各电参数明显增大,BaCl_2引起心肌细胞停搏的阈浓度亦增高。提示Zn对培养的心肌细胞氧自由基所致的损伤具有保护作用。

In present study, the injury induced by oxygen free radical in cultured cardiac cells was observed by adding xanthine and xanthine oxidase into the medium The effects of zinc on the injury of cells were also observed, taking the electro physiological changes and threshold concentration of BaCl_2 which induces cell stop beating as index. The results showed that the electric parameters of action potential (the amplitude, overshoot, maximum diastolic potential, threshold, maximum rate of depolarization and duration of action potential ) were decreased in XOD group, compared with the control. The threshold concentration of BaCl_2 was decreased too. However, there were no significant differences between Zn group and control group except the TP value. The electric parameters of action potential was increased notab(?)y in Zn group, compared with XOD group. Furthermore, the threshold concentration of BaCl_2 in Zn group was increased too. It is suggested that Zn possessed the protective effects on cultured cardiac cells from the injury induced by oxygen free radical.