树鼩局部脑缺血模型的复制及缺血后腺苷酸、钙和MDA等的变化

Production model of focal cerebral ischemia and adenylic acid,calcium and MDA changes following focal cerebral ischemia in tupaiidae

多数学者认为树鼩属低等灵长类动物,关于树鼩局部脑缺血的研究尚未见报导。用电凝阻断树鼩一侧大脑中动脉(MCA-O)的方法复制局部脑缺血模型。发现血管闭塞15分钟及6小时后,缺血区rCBF分别为凝闭前的36.74%和39.84%,SEP分别为凝闭前的21.83%和36.78%;血管闭塞6小时后,皮层组织ATP含量减少了5.2倍,AMP含量增加了4.4倍;钙显著强多,MDA含量增加。形态学检查表明发生了局部缺血性脑梗塞。结果说明了能量衰竭,脂质过氧化物的形成,Ca^(2+)内流增加在脑缺血损伤发病中起重要作用。作者认为,由于树鼩的生物学特性,较其它哺乳动物更适合局部脑缺血的实验研究。

Tupaiidae has been considered as low primate by most scholars. Focal cerebral ischemia study on tupaiidae has not been reported. Acute focal cerebral ischemic model in tupaiidae was produced by cauterizing the middle cerebral artery. The results indicated that after 15 minutes and 6 hrs occlusion of the middle cerebral artery, the rCBF's in the ischemic cortex were reduced respectively to 36.7% and 39.8% of those before occlusion, and the amplitudes of SFP to 21.83% and 36.78%. After 6 hrs of ischemia, a decrease in ATP content to 5.2 times that of the control, and an increase in AMP to 4.4 times were measured. Calcium and MDA contents in ischemic cerebral cortex increased (P<0.01, P<0.05, respectively). Morphologically damage was demonstrated in the ischemic cortex. These results indicated that energy depletion, lipoperoxide formation and the increase of calcium influx may play an important role in pathogenesis of cerebral ischemia. The aut hors suggest that, for its biological characteristics, tupaiidae may be more suitable for research of focal cerebral ischemia experiments than other mammal animals.