自由基清除酶(剂)对SMAO休克大鼠保护作用的研究

Protective effects of oxygen free radical scavengers during SMAO shock in rats

夹闭大鼠肠系膜上动脉1h后松夹,于松夹前分别给予超氧化物歧化酶(superoxide dismutasv, SOD)、过氧化氢酶(catalase, CAT)、别嘌呤醇(allopurinol, ALLO)、SOD+CAT、SOD+ALLO和SOD+CAT+ALLO。结果表明,除单用CAT外,其他单用或伍用自由基清除酶(剂)均有抗脂质过氧化损伤效应,表现为应用自由基清除酶(剂)能明显抑制肠、心、肝、肺组织中丙二醛(malodialdehyde, MDA)含量的升高,稳定组织内源性SOD的活力,同时血浆溶酶休酶的释放减少,休克动物血压升高,死亡率下降,平均存活时间明显延长。实验结果尚表明,应用自由基清除酶(剂)可以明显减轻肠系膜上动脉夹闭(superior mcsenteric artery occlusion, SMAO)休克大鼠小肠粘膜的病理改变。

After one hour occlusion of superior mesenteric artery in rats and just before releasing the clamp, SOD (superoxide dismutase), CAT(catalase), ALLO(allopurinal), SOD+CAT, SOD+ALLO, SOD+CAT+ALLO were administrated seperately to the animal. The results showed that all oxygen free radical scavengers, except CAT, could alleviate tissue lipid peroxidation injury, depressed the accumulation of MDA (malondialdehyde)in bowel, heart, liver, and lung tissues, stabilized the activities of endogenous SOD, decreased the release of lysosome enzymes, elevated the blood pressure and prolonged the survival time of shocked animals. The results also showed that administration of oxygen free radical scavengers could alleviate the instestinal mucosal lesion in SMAO (superion mesentetic artery occlusion) shock.