针刺家兔不同部位对白细胞致热原性发热及其脑脊液环核苷酸含量的影响

Observations on the changes of cAMP,cGMP levels in CSF during conscious rabbits with LP fever given acupuncture on main points

本文应用白细胞致热原(LP)复制家兔发热模型,并针刺其相当于“督脉经”上的三个不同部位降热时,测定家兔脑脊液(c.s.f)中环核苷酸含量,实验结果发现:LP性发热家免c.s.f中cAMP含量明显高于正常对照组(正常对照组cAMP含量为18.60±1.56 pmol/ml,LP组为25.30±1.14pmol/mlP<0.01),cGMP含量则低于空白对照组(空白对照组值为14.10±1.33pmol/ml)LP组值为8.95±0.72pmol/mlP<0.05)。三个针刺“穴位”LP组针刺后体温高度明显受抑制,其c.s.f中cAMP禽量亦明显低于LP组(LP组cAMP含量25.3)±1.14 pmol/ml,三个针刺“穴位”LP组cAMP含量分别为15.00±1.37;17.31±2.27;和16.09±1.35pmol/ml);cGMP含量则高于LP组(LP组cGMP含量为8.95±0.72pmol/ml,三个针刺“穴位”LP组cGMP含量分别为:14.68±0.86;13.93±0.83和16.70±1.95pmol/ml)。表明针刺“督脉经”上三个部位均有明显降热作用,但针刺“封穴”LP组,针刺“穴位”正常组及针刺“非穴”LP组均未发现有抑制体温升高的作用,对c.s.f环核苷酸含量亦无明显影响。我们推理;针刺发热家兔的三个不同部位降热效应机制,可能是针刺该部位的神经解剖装置发出冲动并可能通过神经传至中枢并使环核苷酸含量变化,从而达到降热目的的。

When the fever was induced by leukocytic pyrogen(LP)on rabbits, we observed the effects of acupuncture on the main point of 'Du Channel'. At the same time measured the changes of cAMP and cGMP levels in CSF of the animals.It was found that cAMP levels increased (cAMP levels of normal group 18.60±1.56 pmol/ml and experimental group 25.30±1.14pmol/ml,P<0.01), but cGMP levels decreased(normal group 14.10±1.33pmol/ml;experimental group 8.95±0.72 pmol/ml). When the fever was induced by LP on conscious rabbits,As three points of 'Du Channel' were given acupuncture,signifi cant febrifugal effects occured and cAMP levels increased(16.79±1.95 pmol/ml).However when other points were acupunctured neither febrifugal effects nor cAMP, cGMP levelse change in CSF. were observed. We deduced that the febrifugal effects of main acupunctur points of'Du Channel' were caused by changes of cAMP cGMP levels in CSF of rab bits.