内皮素在止血带休克发病学中的意义

Pathogenetic significance of endothelin in rat tourniquet shock

本文在大鼠止血带休克模型上观察血浆内皮素水平变化,和内皮素抗血清的治疗作用。结果发现,经止血带结扎双后肢4小时的动物松解结扎后,发生进行性低血压,血浆内皮素水平升高(5.9±0.4pg/ml vs.假手术组动物2.3±0.2 pg/ml,P<0.01)TXB_2含量增加,组织蛋白酶-D和乳酸脱氢酶活性增强,骨骼肌线粒体钙超载等一系列损伤性变化。应用内皮素抗血清治疗,可有效地减轻上述变化。提示,内皮素在肌肉缺血—再灌注损伤中具有重要发病学意义,可能是创伤性休克的重要发病因素之一。

The changes of plasma endothelin (ET) level of the tourniquet shocked rats, and the therapeutic effects of ET-antiserum were observed. It was found that after releasing the tourniquet severe tissue damage was found in rats subjected to ligature hindquarter for 4hr: progressive hypotension, elevation of plasma ET level (5.9±0.4 vs 2.3±0.2 Pg /ml in sham group, P<0.01), enhancement of plasma TXB_2 content, increase in the activities of cathepsin D and lactate dehydrogenase, and overload of calcium in skeletal muscular mitochondria. However treatment of the tourniqueted rats with ET-antiserum could significantly alleviate tissue damages mentioned above. The results showed that ET might play a pathogenetic role in the muscular ischemia reperfusion injury, and it should be considered one of the important factors inducing traumatic shock.