大鼠肺表面活性物质磷脂在急性肺泡损伤时的变化

Changes of surfactant phospholipids in acute alveolar injury of the rats

以6mg/kg体重皮下注射N-亚硝基-N-氨基甲酸乙酯诱发大鼠急性肺泡损伤,在注射后第2天,肺泡灌洗液(即肺泡空间成份)表面活性物质磷脂含量明显减少,而蛋白质含量明显增加。细胞内层状体和微粒体中磷脂及蛋白质含量未见明显改变。与此同时,各肺成份中磷脂的组成也发生了变化,即PG/PI的比率明显降低。分子种的检测显示,两种酸性磷脂(Po和PI)的分子种构成不同,其主要分子种在急性肺泡损伤时有所改变。

Acute alveolar injury was induced in rats by subcutaneous injection of Nnitroso-N-methylurethane (NMU) 6mg/kg body wt. NMU decreased the content of surfactent phosphoLipids, and increased the protein of alveolar lavage return of rats after 2 days of the injection. Such changes were not observed in lamellar bodies and microsomcs. Composition of phospholipids also changed in all lung fractions it showed that the ratio of PG/PI was decreased significantly in response to NMU. The molecular species profiles of both acidic phospholipids in the lung fractions were distinctly different from each other and the main molecular species were attered during the acute alveolar injury.