心肌缺血的膜损伤及蝮蛇抗栓酶修饰效应的实验研究

An experimental study of myocardial membrane injury in isoproterenol-induced myocardial infarction and the modulative effects of Svate

本实验以异丙肾上腺素性心肌缺血(ISP-MI)为模型,对心肌膜脂、膜酶及自由基系统三个方面进行了研究,旨在探讨蝮蛇抗栓酶(Svate)对膜损伤的修饰作用。结果发现,大鼠皮下注射异丙肾(ISP,85mg/kg)24h后,心肌膜脂、膜酶及自由基代谢发生明显紊乱,标志着膜损伤的存在。舌下静脉注射Svate(0.25U/kg)治疗组,与单纯缺血组比较,心肌线粒体Ch/PL比降低,膜脂流动性明显恢复;心肌膜脂过氧化产物丙二醛(MDA)下降,谷胱甘肽过氧化物酶(GSH-Px)活力增加,Na^+-K^+-ATPase活力明显升高及Ca^(2+)-Mg^(2+)-ATPase活力恢复正常。以上结果说明,Svate对缺血心肌膜损伤有一定的修饰效应。

Changes in lipids, enzymes and free-radical system of myocardial membranes were investigated in isoproterenol-induced myocardial infarction so as to study the modulative effects of Svate on myocardial membrane injury. Animals were killed 24h after ISP(85mg/kg) injection. The results suggested that disturbances in lipids metabolism, enzymes and free-radical system in ischemic myocardium, indicating the existence of myocardial membrane injury. In the Svate(0.25U/kg)-treated group, a beneficial effect of Svate exerted on membrane injury, as indicated by increased activities of GSH-Px, SOD and Na^+K^+-ATPase, and decreased Ch/PL ratio, MDA contents and Ca^(2+)-Mg^(2+)-ATPase activities.