胰岛素依赖型糖尿病患者外周血单核细胞膜表面HLA-Ⅱ类抗原、白细胞介素2受体和铁传递蛋白受体的表达

Expression of HLA class Ⅱ antigens, interleukin-2 receptors and transferrin receptors of monocytes from patients with insulindependent diabetes mellitus

本文作者用间接免疫荧光法检测了10例胰岛素依赖型糖尿病(IDDM)患者外周血单核细胞膜表面HLA-DR、DQ、DP抗原、白细胞介素2受体(IL-2R)、铁传递蛋白受体(TfR)的表达。术中应用了相应的单克隆抗体和FITC标记的第二抗体。结果表明,IDDM患者单核细胞膜表面HLADQ抗原、IL-2R和TfR的表达明显高于正常对照组,而HLA-DR、DP抗原的表达在IDDM组与正常对照组之间差异无显著性。推测HLA-DQ、IL-2R和TfR的异常表达在IDDM的发病机制中可能起一定作用。

Expression of HLA class Ⅱ antigens(HLA-DR, DQ and DP), interleukin2 receptors(IL-2R) and transferrin receptors(TfR) of blood monocytes from 10 patients with insulin-dependent diabetes meIlitus (IDDM) were assayed with the indirect immune fluorescence technique using corresponding monoclonal antibodies and the FITC-labelled second antibody. The results showed that the number of HLA-DQ^+ monocytes was much more in diabetics than in normal controls. The percentages of HLA-DR^+ and HLA-DP^+ monocytes in diabetics were not different significantly from those in normal controls. Besides, IL-2R^+ and TfR^+ monocytes were also found to be very much increased in diabetics as compared with controls. It was possible that increased expression of HLA-DQ antigen, IL-2R and TfR of monocytes in patients with IDDM might play a role in the pathogenesis of the autoimmune reaction.