70%氧对大鼠肺切除后肺增生的影响

Effect of 70% oxygen on postresectional lung growth in rats

为检验高浓度氧对肺切除后肺增生的影响,将大鼠右肺3个叶切除后置于空气或70%氧箱中,2周后行左肺电镜及显微测量学观察。开胸而不切除肺者作为对照组。肺切除后Ⅰ、Ⅱ型肺泡上皮细胞、毛细血管内皮细胞、间质细胞和基质各总体积均较对照组增加100～200%;毛细血管和Ⅰ型上皮总表面积各增40%。吸70%氧对肺增生无显著抑制作用,但较单纯肺切除组Ⅱ型上皮和间质细胞体积密度各增加78%和54%,Ⅱ型上皮和成纤维细胞显著增多,出现间质水肿、中性白细胞聚集和Ⅱ型上皮线粒体异常。作者认为70%氧对大鼠肺切除后肺增生无显著影响,但并非完全无害。

We tested the hypothesis that exposure to hyperoxia could inhibit postresectional compensatory lung growth. We removed 3 upper lobes of the right lung of rats allowed them to breathe either air or 70% oxygen after operation, and made electronmicroscopy and morphometry on the left lung 14 days after the resection. Rats that had a thoracotomy without removal of lung were used as controls. Resection of lung resulted in increased of total volume of alveolar type 1 and type 2 cells, capillary endothelial cells, interstitial cells and matrix to about 100～200%. Exposure to 70% oxygen did not inhibit postresectional growth signifieantiy, but it did result in a 780% greater alveolar type 2 cell and a 54% greater interstitial cell volume densities. The type 2 cells and fibroblasts appeared to be increased and interstitial edema and neutrophil accumulation were also present. We concluded that 70% oxygen did not inhibit postresectional lung growth but it was not totally harmless.