清热复方对热休克大鼠肝、肺组织热休克蛋白基因表达的调控作用

Regulatory Effect of Prescriptions for Clearing Away Heat on Gene Expression of Heat Shock Proteins in Liver and Lung Tissues of Heat Shock Rats

[目的]探讨清热复方对大鼠热休克蛋白 70(HSP70)表达的调控作用。[方法]将 70只大鼠随机分为热休克模型组、热休克加白虎汤组、热休克加黄连解毒汤组、内毒素模型组、内毒素加白虎汤组、内毒素加黄连解毒汤组和正常对照组7组,每组10只。采用逆转录聚合酶链反应(RT-PCR)对热休克模型组、内毒素模型组和正常对照组大鼠的肝、肺组织进行了HSP70 mRNA表达的检测,观察清热复方白虎汤和黄连解毒汤分别对两种模型大鼠肝、肺组织 HSP70 mRNA表达的影响。【结果】热休克模型组、热休克加白虎汤组和热休克加黄连解毒汤组中HSP70 mRNA的表达高于正常对照组(P<0.05);热休克加白虎汤组和热休克加黄连解毒汤组均高于热休克模型组(P<0.05);内毒素模型组、内毒素加白虎汤组和内毒素加黄连解毒汤组之间的HSP70 mRNA表达以及与正常对照组相比均无显著性差异(P>0.05)。[结论]提示不同因素导致的热证状态下HSP70的基因表达不一致,清热复方可诱导热休克大鼠肝、肺组织中HSP70表达的增加,从而提高细胞的耐受力,避免细胞组织的损伤。

[Objective] To explore the regulatory effect of compound prescriptions for clearing away heat on gene expression of heat shock protein 70 (HSP70) in liver and lung tissues of heat shock rats. [Methods] Seventy rats were randomized into 7 groups: A (models of heat shock), B (heat shoek models treated with Baihu Decoction), C (heat shock models treated with Huanglian Jiedu Decoction), D (endotoxin-induced models), E (endotoxin-induced models treated with Baihu Decoction), F (endotoxin-induced models treated with Huanglian Jiedu Decoction) and G (normal control group). mRNA expression of HSP70 in the liver and lung tissues of all the groups were detected by reverse transcription polymerase chain reaction (RT-PCR) and the influence of Baihu Decoction and Huanglian Jiedu Decoction on the expression of HSP70 were observed. [Results] The expression of HSP70 in Group A, B and C was higher than that in Group G (P<0.05); HSP70 expression in Group B was higher than that in Group A (P <0.01 ); HSP70 expression in liver of Group B was higher than that in Group C (P <0.05 ); the differences of HSP70 expression between Group D, E, F and Group G were insignficant(P>0.05). [Conclusion] HSP70 expression varies in heat syndrome induced hy various factors; Baihu Decoction and Huanglian Jiedu Decoction can increase the expression of HSP70 in liver and lung tissues of heat shock rats and thus increase the heat tolerance of cells and prevent the cells from damaging.