解毒利肺口服液对病毒性肺炎小鼠免疫功能的影响

Effect of Jiedu Lifei Oral Liquid on Immune Function in Mice with Viral Pneumonia

[目的] 研究解毒利肺口服液(简称 JLOL,主要由金银花、连翘、黄芩、川贝等组成)对感染病毒机体免疫系统的影响。[方法] 60只小鼠随机分为5组:正常对照组、模型对照组、病毒唑组、JLOL大剂量组及JLOL小剂量组。运用流感病毒鼠肺适应株(FMI)感染小鼠制成病毒性肺炎模型,观察JLOL对病毒性肺炎小鼠血浆中细胞因子白细胞介素l(IL-l)、白细胞介素2(IL-2)、肿瘤坏死因子α(TNF-α)和干扰素γ(IFN-γ)水平的影响。[结果] 模型对照组与正常对照组比较,IL-l及IFN-γ水平降低,TNF-α水平升高(均P<0.01),IL-2虽有降低趋势,但差异无显著性(P>0.05)。用JLOL治疗后,血浆中IL-2及IFN-γ水平升高,TNF-α水平降低(P<0.05或P<0.01);IL-l水平有升高趋势,但差异无显著性(P>0.0)。[结论]该药能提高病毒性肺炎小鼠IL-2及IFN-γ水平从而增强机体抗病毒感染的免疫功能,并通过抑制TNF-α的过量产生,减轻免疫性病理损害。

[Objective] To observe the effect of Jiedu Lifei Oral Liquid (JLOL, mainly composed of Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae, Bulbus Frityllariae, etc. ) on the immune function in mice with viral pniumonia. [Methods] Sixty mice were randomized into 5 groups: normal group (A), model group (B), virazole group (C), small- dose JLOL group (D) and large-dose JLOL group (E). Mouse models of viral pheumonia were induced by infection of influenza virus FM1. Effects of JLOL on serum levels of interleukin-1 (IL-1), interleukin-2 (IL-2), tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ) were observed. [Results] Compared with normal group, serum levels of IL- 1 and (IFN-γ were decreased and TNF-α increased (P <0.01) and IL-2 was also decreased (P >0.05 ) in model group. After treatment wth JLOL, serum levels of IL-2 and IFN-γ were increased , TNF-α decreased (P <0.01 or P <0.05 ) and IL-1 wns increased (P >0.05). [ Conclusion] JLOL can increase the immune function by increasing the serum levels of IL2 and IFN-γ and can reduce the immune impairment by inhibiting the over production of TNF-α.