MTHFR基因多态性与妊高征的遗传易感性研究

Polymorphisms of MTHFR gene and genetic susceptibility of pregnancy induced hypertension.

目的 :探讨N5,N10 亚甲基四氢叶酸还原酶 (MTHFR)基因多态性与妊高征(PIH)的关系。方法 :运用多聚酶链反应 限制性内切酶片段长度多态性 (PCR RFLP)技术检测 99例PIH及 5 4例对照组的MTHFR基因多态性。结果 :PIH患者的MTHFR基因CC、CT、TT基因型频率分别为 5 3.5 %、31.3%、15 .2 % ,对照组的CC、CT、TT基因型频率分别为 4 6 .3%、4 4.4 %、9.3% ,两组的CT基因型频率差异有显著性 (P <0 .0 5 ) ,CT基因型与重型PIH有关。结论 :MTHFR基因CT基因型可能是PIH的危险因子 。

Objective:To study the relationship between polymorphisms of methylenetetrahydrofolote reductase (MTHFR) and pregnancy induced hypertension (PIH).Method:The polymorphisms of MTHFR gene in the peripheral white blood cells of 99 patients with PIH and 54 controls were analyzed using PCR-RFLP.Result:The frequencies of CC,CT,and TT genotypes of MTHFR gene were 53.5%,31.3% and 15.2% respectively in PIH patients,and were 46.3%, 44.4% and 9.3% respectively in the controls.There was significant difference between CT genotypes in PIH patients and those in controls.CT genotype was associated with serious PIH.Conclusion:CT genotypes may be the risk factors of PIH and correlates with serious PIH.