国产红葡萄酒对食饵性AS血管壁NF-κB活化及MCP-1表达的影响

Effect of Chinese Red Wine on Atherosclerosis in Activated Nuclear Factor-κB and Expression of Monocyte Chemotactic Protein-1

目的探讨国产红葡萄酒(RW)在细胞、分子、基因调控水平的抗动脉粥样硬化(AS)作用 ,从而为RW防治AS提供实验佐证。方法采用病理技术、电泳迁移率改变分析法(EMSA)和原位杂交术检测食饵性AS血管壁的病理变化、NF -κB活化及MCP -1mRNA表达情况及RW对其干预的影响。结果与食饵性AS组各相应的时相点相比国产红葡萄酒可显著抑制不同阶段AS血管组织的增生、组织细胞NF -κB活化、显著下调其MCP -1mRNA的表达 ,并具有时间依赖性 ,RW干预12周时作用最强。结论提示国产RW可能是通过抑制NF-κB活性 ,减少MCP -1mRNA表达 ,从而阻抑了AS组织的损伤 ,延缓病变的发展。

Objective In order to study effect of Chinese red wine on experimental atherosclerosis,we studˉied activation of nuclear factor-κB and expression of monocute chemotactic protein_1in different stages of experiˉmental atherosclerosis.Methods Using pathological methods,electrophoretic mobility shift assay(EMSA),and in situ hybridyzation,we study pathological alteration,activity of NF-κB and the expressions of monocyte chemotactic protein_1(MCP-1).Results Chinese red wine could significantly suppress prolifreration of atherosclerosis intima,the NF-κB activation,down_regulate the expressions of MCP_1.Conclusions The results confirmed that Chinese red wine protected AS tissues and prolonged its development by suppressing NF-κB activation,down_regulating the expressions of MCP-1,which may take part in pathogenesis of AS.