硒锰的相互作用对体外培养心肌细胞影响的初探

Preliminary study of the interaction between Se and Mn affected on cultured cardiac cells in vitro

为了探讨不同浓度的硒、锰对体外培养的心肌细胞造成损伤的途径。在进行大鼠胎儿心肌细胞培养时,将牛胎儿血清进行透析后人工添加不同浓度硒、锰到组织培养液中进行细胞的继代培养。其中添加常硒常锰组和未透析血清组的硒、锰含量相同。而低硒、高锰均指所添加硒、锰浓度是常硒常锰的100倍。实验结果表明:只有用透析血清和在透析血清里加低硒,高锰时进行培养的心肌细胞中超氧化物阴离子含量明显高于未透析血清组和添加常硒、常锰组 P<0.05,而 G/SH-Px 活性低于添加常硒常锰组。此外其他各组间无明显差异。从而阐明低硒、高锰能使心肌细胞培养过程中产生自由基,引起心肌细胞损伤。来进一步阐明低硒高锰与克山病发病的关系。

In order to study the way of cardiac lesions,We cultured the rat em-bryonic cardiac cells in the medium which was added different concentrationof Se and Mn to further study the relation between Low-Se and high-Mn andKeshan disease.The result suggested that superoxide anions of cardiac cells cultured bydialysis serum and dialysis serum added low-Se and high Mn were respec-tively higher than that of ordinary level of Se and Mn (P<0.05),and theformer activities of GSH-Px were markedly lower than that of the latter,Butthe difference among other groups was not significant.In conclusion,Se-deficiency and Mn-adeguate can not only produce freeradical in the process of cultured normal cardiac cells but also induce cardiaclesions.