FI 抗原应用于腺鼠疫患者临床早期诊断的研究

Application of FI antigen testing to early clinical diagnosis of patients with Bubonic plague

为解决鼠疫 FI—Ag 的定量检测,本文首先建立了 ELISA 检测系统,该系统灵敏度为3—8ng/ml,并有较高的精密度和准确度,特异性强,与 RPHA 呈现良好的相关性。在此基础上,本文重点研究了链霉素治疗前后鼠疫实验感染动物腺肿液 FI—Ag 的出现、含量、维持时间等。研究结果表明,腺肿FI—Ag 比血清 FI—Ab 产生早(3—4天)而充分(阳检率达100%)。经链霉素治疗,腺肿液细菌培养阳性率仅为28%。而 FI—Ag 仍有80%以上的阳性。因此,通过腺肿液 FI—Ag 的检测途径,为腺鼠疫患者的诊断可达早、快、准、敏的目的。本文还对检测腺肿 FI—Ag、血清抗体以及细菌分离之间的关系进行了讨论。

In order to solve the quantitative determination of plague antigen,we established ELISA testing system first-ly.Sensitivity of the system was 3—8ng/ml,its precision,accuracy and specificity were high,and it had a good correla-tion with RPHA.Based on these,this study was focused on FI-Ag occurrence,aontent and its maintenance before andafter treatment with streptomycin.The results showed that the production of lymph FI-Ag were earlier(3—4days)andmore complete(positive rate was 100%)than that of a erum antibodies.After treatment with streptomycin,the positiverate of bacteria from cultured lymph fluid was less than 28%,but the positive rate of FI-Ag was more than 80%Therefore,it might provide an earlier,faster,more accurate and sensitive method which can be used in the diagnosis ofpatientswith bubonic plague by means of FI-Ag determination in the lymph fluid.The paper also discussed the issue onthe testing of FI-Ag and serum antibodys as well as the isolation of the bacteria.