灰树花的深层培养工艺及其影响因素的研究

Technology of submerged culture of Grifola frondosa and its influence factors

利用发酵罐进行灰树花的深层培养,以期为深层培养技术在灰树花上的应用提供理论依据,为灰树花产品的开发奠定基础。结果表明,最佳的培养条件:发酵温度25℃、起始pH值6.5、搅拌转速150r/min、接种量10%。pH值和通气量对菌丝的生长有极显著的影响,pH值在5.50～6.50,通气量体积比(V/V)在1.00～1.40时,菌丝生物量积累最多;通过营养分析认为,可以通过深层培养进行灰树花产品的研究和开发。

Grifola frondosa was cultured by fermentor. This research is expected to offer some academic bases for submerged culture technology in Grifola frondosa, and to establish material basis for exploitation of Grifola frondosa. The results showed that the optimal conditions of submerged culture ascertained were 25℃ of fermentation temperature, 6.5 of the beginning of pH value, 150 r/min of the rotating speed and 10% of putting in volume. In addition, pH value at 5.50~6.50 and the volume ratio of flow of air-compress at 1.00～1.40, the biomass of mycelium reached the highest level. By nutrition analysis, it is considered that the products of Grifola frondosa may be researched and exploited by submerged culture.