HPLC法测定血清中厄贝沙坦的含量

Content Determination of Irbesartan in Serum by HPLC

目的 :采用高效液相色谱法测定血清中厄贝沙坦的含量。方法 :用 μBondparkC18(7.8mm× 30 0mm)色谱柱 ,以水 甲醇 磷酸为流动相 ,邻苯二甲酸二丁酯为内标 ,流速为 1.2ml·min-1,用紫外检测器于 2 4 5nm波长处检测。结果 :线性范围0 .5～ 2 0 0 μg·ml-1(r =0 .9996 ) ;平均回收率 (95 .3± 6 .3) % ;检测限为 0 .0 5 μg·ml-1。结论 :本法具有操作简便 ,分析快速准确 ,干扰小等优点。

Objective: A HPLC method was established for the content determination of irbesartan in serum.Method: lrbesartan can he separated on μBondpark C 18 ( 7.8 mm×300 mm)column with a mixture of water, methanoland phosphoric acids was mobile phase and detecting wavelength was at 245 nm. The flow rate was 1.2 ml·min -1.Result: The standard curve was linear in the concentration range was within 0.5-200 μg·ml -1 and the correlation coefficient was 0.999 6.The average recovery were 95.3± 6.3%, respectively.The detection limit was 0.05 μg·ml -1.Conclusion: The method is simple, rapid accurate, and reliable.