分光光度法测定血清中的总蛋白含量

Spectrophotometric Determination of Total Proteins in the Serum with Water Soluble Aniline Blue

在 p H=2 .36的 Britton- Robinson缓冲介质中 ,紫色的水溶苯胺蓝与蛋白质形成蓝紫色的结合物 ,其最大吸收波长 λmax为 688nm,比水溶苯胺蓝试剂本身红移 88nm,表观摩尔吸光系数达4.2 5× 1 0 5L/( mol· cm) (与牛血清白蛋白结合 )。制定了 4种蛋白质的标准曲线 ,用于人血清样品中总蛋白的测定 。

It was found that water soluble aniline blue could react with proteins forming complexes in Britton-Robinson buffer at pH 2 36 and room temperature, which gives maximum absorption peak at 688 nm with 88 nm red shift in contrast with water soluble aniline blue. A molar absorptivitity of 4 25×105 L/(mol·cm) for BSA was determined. Four standard curves for proteins such as BSA, HAS, γ-G and casein were constructed under the optimum conditions. The analytical method was proposed to determine the content of total proteins in human serum samples with satisfactory results and in agreement with that of bromocresol green method.