5种抗真菌药物的体外敏感性试验

The susceptibilities test in vitor of 5 species of antifungal drugs

目的:了解呼吸道感染患者痰中念珠菌对5种抗真菌药物的敏感性特性,为临床合理用药提供依据。方法:采用标准微量稀释法(NCCLSM-27A)测定43珠念珠菌对氟康唑片、伊曲康唑胶囊、大蒜素注射液、大蒜液及氟康唑注射液的最低抑菌浓度(MIC)。结果:氟康唑片MIC为0.8～100μg/mL,83.7％(36/43)敏感,氟康唑注射液MIC 6.25～25μg/mL,100％敏感;伊曲康唑胶囊2.5～10μg/mL,90.7％(39/43)敏感;大蒜素注射液4.69～18.75μg/mL,100％敏感;大蒜液(本院制剂)0.78～100mg/mL,88.4％(38/43)敏感。结论:结果提示对白色念珠菌引起的深部感染患者,12.5μg以上氟康唑注射液、大蒜素注射液,临床治疗有效。

Objective: To study the susceptibilities in vitro of the Candida collected from sputum of respiratory tract infectious to 5 species of antifungal drugs, and offer reference for the clinical treatment. Methods: The MIC of 43 strains of Candida to fluconazole tablets fiuconazole injectio, intraconazole capsules, allimin injectio were tested by broth microdilution methods according to the National Committee of Clinical Laboratory Standard Recommendations. Results: The MIC of fluconazole was 0.8～100μg/mL and 83.7%(36/43) was susceptible; The MIC of fluconazole injectio 6.25～25μg/mL and 100% was susceptible, itraconazle capsules 2.5～10μg/mL and 90.7% was susceptible, allimin injectio 4.69～18.75μg/mL and 100% was susceptible; allimin liquid 0.78～100mg/mL and 88.4% were susceptible. Conclusion: We can infer that it was effective for the deep infectious patients for Candida with 12.5μg or more dose of fluconazole injectio or allimin injectio.