高糖对视网膜Müller细胞碱性成纤维细胞生长因子表达及钙离子通道的影响

Effects of high concentration glucose on bFGF expressionand calcium channels of retinal Müller cells cultured in vitro

目的 :观察在高浓度葡萄糖条件下体外培养兔视网膜 Müller细胞碱性成纤维细胞生长因子 (basicfibroblast growth factor,b FGF)的表达及其钙 (Ca2 + )通道的变化。方法 :采用免疫细胞化学及透射电镜方法鉴定体外培养的兔视网膜 Müller细胞 ;应用免疫细胞化学方法定性观察高糖条件下视网膜 Müller细胞 b FGF表达的变化 ;利用膜片钳技术观察高糖条件下 Müller细胞 Ca2 + 通道的变化。结果 :高糖可以刺激视网膜 Müller细胞表达b FGF,对 Ca2 + 通道无明显影响。结论 :高糖刺激 Müller细胞 b FGF的表达 ,而发挥促血管生成的作用 ,在此过程中 Ca2 +

ObjectiveTo observe the effects of high concentration glucose on bFGF expression and calcium channels of rabbit retinal Müller cells cultured in vitro . MethodsTransmission electron microscopy and immunocytochemical staining method were used to identify Müller cells. The changes of bFGF expressed by Müller cells were studied with immunocytochemical method in a qualitative way. Patch clamp technique was used to observe the changes of calcium channels caused by high concentration glucose. ResultsbFGF expression was enhanced obviously by high concentration glucose. Obvious changes of calcium channels were not found. ConclusionbFGF expression secreted by cultured Müller cells in vitro can be enhanced at high concentration glucose. Müller cells play a potential role in neovascularization of diabetic retinopathy. Obvious changes of calcium channels are not found during the process.