立体定向大鼠脑内SHG-44人脑胶质瘤模型的建立

AEstablishment of SHG-44 human glioma model in brain of Wistar rat with stereotactic technique

目的 :探讨建立大鼠 SHG- 4 4脑胶质瘤模型的方法。方法 :选用雄性 Wistar大鼠 ,接种前连续 3d用地塞米松 1 mg/ 1 0 0 g体重灌胃 ;在立体定向条件下选取大鼠脑右侧尾状核区为靶点 ,接种 2× 1 0 5个处于对数生长期的 SHG- 4 4细胞 ,接种后观察大鼠生长状态 ,分别于第 1、 2周进行核磁共振 (MRI)检查。在实验第 2周时解剖标本 ,行组织病理和 GFAP免疫组化检查。结果 :接种 1周后核磁共振检查 ,脑内形成实体瘤 ;HE染色组织病理证实是胶质瘤 ,GFAP免疫组化阳性 ;成瘤率约 6 0 %。结论 :用预先免疫抑制的方法 。

ObjectiveTo establish solid intracerebral human glioma model in Wistar rat with xenograft methods. MethodsThe SHG 44 cells were injected into brain right caudate nucleus of previous immuno inhibitory Wistar rats with stereotactic technique. The MRI scans were performed at 1 week and 2 weeks later after implantation. After 2 weeks the rats were killed and pathological examination and immunohistologic stain for human GFAP were used. ResultsThe MRI scan after 1 week of implantation showed the glioma was growing,pathological histochemical examination demonstrated the tumor was glioma. Human GFAP stain was positive. The growth rate of glioma model was about 60%. ConclusionSolid intracrebral human glioma model in previous immuno inhibitory Wistar rat is successfully established.