β_2肾上腺素能受体基因与小儿哮喘的关系

Association of β_2-adrenergic receptor gene with asthma in children

目的 :探讨β2 肾上腺素能受体 (β2 AR)基因与小儿哮喘的关系。方法 :采用聚合酶链反应 (PCR)技术扩增β2 AR 5′端基因片段 ,对 6 5例哮喘患儿和 2 0例正常对照进行分析。结果 :哮喘组中 2 2例及对照组中 6例存在β2 AR基因片段的缺失 ,缺失率分别为 33.8% (2 2 / 6 5 )和 30 % (6 / 2 0 ) ,哮喘组与对照组相比差异无显著性 ,但哮喘组中重度与轻度、中度比较 β2 AR基因缺失率高。结论 :β2 AR基因片段的缺失与哮喘发病无关 ,但与重度哮喘患者的发病有关。

ObjectiveTo investigate the association of beta 2 adrenergic receptor (β 2AR) gene with asthma in children. MethodsThe 5′ 234 bp fragment of β 2AR gene was analyzed by PCR method. ResultsThe fragment deletion of β 2AR existed in 22 cases of 65 asthmatic patients and in 6 cases of 20 healthy clildren in control group, the deletion rates were 33 8% and 30%,respectively . There was no significant difference between the two groups. The deletion rate of β 2AR gene fragment in severe asthma group was higher than that in mild moderate asthma group. ConclusionThe deletion of this fragment of β 2AR gene isn′t related to onset of asthma, but it is related to onset of severe asthma.