摘要
比较 GM- CSF+IL- 4与 IL- 3+IL- 4两种方法培养制备的树突状细胞 (Dendritic cell,DC)在形态、产量、免疫表型和抗原摄取能力方面的差异。用 GM- CSF+IL- 4和 IL- 3+IL- 4分别诱导小鼠骨髓来源的前体细胞分化为成熟树突状细胞 ,通过相差显微镜、扫描电镜、激光共聚焦扫描显微镜进行形态观察 ,流式细胞术分析细胞免疫表型和摄取抗原能力。结果表明 ,IL- 3+IL- 4诱导的 DC产量高 ,细胞纯度好 ,形态上与 GM- CSF+IL- 4诱导的DC类似 ;细胞免疫表型显示高表达 MHC 类分子 ,但低表达或不表达 CD80、CD86 ;IL- 3+IL- 4诱导的 DC具有强大的摄取抗原能力。 IL- 3替代 GM- CSF可诱导低表达共刺激分子的耐受型
In order to compare the effects of GM CSF+IL 4 with the effects of IL 3+IL 4 on the differentiation and development of dendritic cells (DCs) from mouse bone marrow, we cultured bone marrow hematopoietic progenitor cell in vitro and examined whether DCs induced by IL 3+IL 4 (IL 3 DCs) differed from the DCs induced by GM CSF+IL 4 (GM CSF DCs) in morphology, yields, phenotype and endocytic activity. Scanning electron microscope, laser scanning confocal microscope (LSCM) and flow cytometer (FCM) were used. The results showed that the DCs induced by IL 3+IL 4 and the DCs induced by GM CSF+IL 4 had similar morphology, and the DCs induced by IL 3+IL 4 expressed high level of major histocompability complex (MHC) class Ⅱ. Besides, IL 3 DCs had the characteristics of higher yields and better consistency. But CD80 and CD86 were expressed at low levels or absent on IL 3 treated DCs. The capability of uptaking antigen of IL 3 DCs was more powerful than that of GM CSF DCs. IL 3 could substitute for GM CSF in culturing DCs from mouse bone marrow cells and could obtain the tolerance DCs that lack costimulatory molecules.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
2004年第2期233-237,共5页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目 (3 0 2 70 5 16
3 0 2 70 5 88)