高效毛细管电泳法测定注射用盐酸头孢吡肟的含量

Determination of cefepime dihydrochloride for injection by HPCE

目的 测定注射用盐酸头孢吡肟的含量。方法 高效毛细管电泳法。毛细管 6 0 cm× 75 μm;运行缓冲液 30 mmol/ L 硼砂 (p H9.2 ) ,高压进样 5 s,分离电压 12 k V,温度为 2 5℃ ,检测波长为 2 5 4 nm,地塞米松磷酸钠为内标。结果 头孢吡肟在 6～ 30 μg/ ml浓度范围内线性关系良好 (r=0 .9998) ,平均回收率为 98.97%。结论 本法简单、快捷、灵敏。

Objective To determine the content of cefepime dihydrochloride for injection. Methods HPCE was used. The optimum condition was achieved with a fuse silica capillary tube (60cm×75μm), 30mmol/L sodium tetraborate decahydrate buffer (pH9.2), at a constant voltage of 12kV and temperature at 25℃. The UV detection wavelength was at 254nm and dexamethasone sodium phosphate was used as internal standard. Results The calibration curves was linear in the range of 6～30μg/ml for cefepime ( r =0.9998). The average recovery rate of cefepime was 98.97%. Conclusion This method is simple, rapid and sensitive.