小批量稻谷种子蛋白质含量的近红外透射光谱分析

Determination of protein content in small batch rice seed by near infrared transmittance spectroscopy(NITS)

以完整水稻种子为样品,利用近红外透射谷物分析仪对186份批量稻谷进行扫描并测定了蛋白质含量的参比数据。采用多种数学计量学处理方法和不同的回归统计方法进行定标曲线的开发和比较,优化得到了小批量水稻种子蛋白质含量测定的近红外定标方程。其定标标准偏差(SEC)、交叉检验标准误差(SECV)、定标相关系数(RSQ)和交叉验证相关系数(I-VR)分别为0.255 8、0.279 5、0.972 8、0.967 5。研究采用整粒小量样品(5 g)来分析,效果较好,可直接用于育种早世代选择。

Using intact rice seeds as test samples, one hundred and eighty-six samples of small batch rice seeds were scanned by near infrared transmittance spectroscopy and their protein contents were determined by reference method. Several mathematics treatments to the sample spectra and several different regression methods were adopted to optimize a good calibration equation for determination of protein content in small batch rice kernel. For the protein content in small batch rice grian. the standard error of calibration(SEC),standard error of cross-validation(SECV),regression squared(RSQ)and 1 minus the variance ratio (1-VR) of the calibration selected were 0.255 8, 0.279 5, 0.972 8 and 0.967 5, respectively. Better results were obtained by using intact rice seeds small sample (5g). So the NITS analysis could be used to screen the early generation lines in rice seeds breeding for quality improvement.