摘要
以阴离子型层状材料——水滑石(LDHs)作为载体,经过戊二醛活化处理后采用共价结合的方法固定化木瓜蛋白酶。研究了溶液酶及固定化酶的催化特性,并进行了比较。结果表明:固定化酶的最适反应pH值为8.0~9.0,最适反应温度为65℃;固定化酶的pH稳定性、热稳定性、对尿素和有机溶剂稳定性、贮藏稳定性与溶液酶相比都有显著的提高,并且固定化酶具有较好的操作稳定性,连续反应11批后,酶活未见下降。
The immobilization of papain using covalent method on the anion layer material-layered double hydroxides (LDHs) were studied. LDHs were activated by glutaraldehyde before being used as the carrier of the immobilized enzymes. The characters of these immobilized papain were also studied and compared with native papain. The results showed that the optimum pH value is 8.0~9.0, the optimum temperature is 65℃; the stabilities of the immobilized enzyme of the pH value, of urea ,of organic solvent and the thermostability, the stored stability are obviously higher than those of native papain, and the operational stability of the immobilized papain is also better than that of native papain, and after 11 cycles batch reactions, the activity of the immobilized papain does not descent.
出处
《食品科技》
CAS
北大核心
2004年第3期16-19,共4页
Food Science and Technology
基金
国家自然科学基金资助项目(29973004)
北京市自然科学基金资助项目(582014)。