红酵母RY-2001产类胡萝卜素的研究

Studies on conditions of carotene production by rhodotorula RY-2001

研究碳源、氮源和通气量对红酵母 (Rhodotorulasp .)RY -2 0 0 1产类胡萝卜素的影响 ,并通过正交试验优化其产类胡萝卜素的培养基组成。结果表明 ,RY -2 0 0 1产类胡萝卜素的适宜培养基 :蛋白胨 4%、葡萄糖 2 %、MgSO40 .1 %、酵母膏 1 %。摇瓶装量为 5 0ml/2 5 0ml,2 8℃培养 48h ,在上述条件下 ,红酵母中类胡萝卜素含量为 81 .465ug/g。

Effect of carbon source, nitrogen source and aeration on carotenoid production of strain Rhodotorula sp.RY-2001 were investigated. The compositions of medium were optimized with orthogonal test . The results showed that the optimal medium was as follows: Peptong4%、glucose2%、MgSO40.1%,yeast extract 1%. The optimal medium volume in 250ml flask was 50ml. 81.465ug carotene/g dry cell were produced by strain RY-2001 with above-mentioned conditions after growing at 28℃ for 48h.