趋化肽fMLP与博安霉素联合对巨噬细胞功能的影响

The effects of chemotactic peptide fMLP-boanmycin combination on macrophage functions

目的 趋化肽对白细胞有趋化和活化作用 ,博安霉素 (Boanmycin ,BAM )对多种肿瘤有治疗作用。趋化肽fMLP(CHO Met Ile Phe,fMLP)瘤周给药可提高博安霉素的抗肿瘤作用。为探讨这种现象的作用机制 ,本实验观察fMLP对博安霉素体内处理的巨噬细胞的趋化作用及其超氧阴离子自由基 (O2 -)释放的影响。 方法 博安霉素注入小鼠腹腔 ,3天后取出其腹腔巨噬细胞。趋化实验采用Transwell模型 ,自由基 (O2 -)测定采用NBT法。 结果 fMLP 4 .6× 10 -7mol/L对BAM 1mg/kg体内处理的巨噬细胞有较好的趋化作用 ,与对照相比 ,有显著差异 (P <0 .0 5 )。fMLP 4 .6× 10 -7mol/L使BAM 1mg/kg、3mg/kg和 10mg/kg体内处理的巨噬细胞释放O2 -的量 ,与对照相比也显著增加 (P <0 .0 1)。 结论 趋化肽fMLP使博安霉素处理的巨噬细胞趋化功能和释放自由基功能增强 ,可能是fMLP提高博安霉素抗肿瘤作用的机理之一。

Objective Chemotactic peptide can activate and attract macrophages. Boanmycin (BAM ) is effective against a panel of cancers in clinical trials. Chemotactic peptide fMLP may enhance BAM antitumor action. This study is set to investigate the chemotactic action of fMLP on macrophages pretreated with BAM and the effect of it on the release of superoxide anion (O 2-)from the macrophages in order to explain the mechanism of above phenomenon. Methods Macrophages were taken out from peritoneal cavity of mice pretreated with BAM by ip route. Chemotactic experiment was carried out by using Transwell model. Superoxide anion was determined by NBT assay. Results fMLP 4.6×10 -7 mol/L had higher chemotactic activity on macrophages pretreated with BAM 1mg/kg (P<0.05). fMLP 4.6×10 -7 mol/L significantly increased the release of superoxide anion (O 2-) from macrophages pretreated with BAM 1mg/kg, 3mg/kg and 10mg/kg (P< 0.01). Conclusion That fMLP makes the chemotactic function and superoxide anion release function of the macrophages pretreated with BAM increase may be one of the mechanisms that fMLP may enhance BAM antitumor action.