三苯氧胺对乳腺癌和宫颈癌细胞增殖的影响

EFFECT OF TAMOXIFEN ON PROLIFERATION OF CULTURED BREAST CANCER AND CERVICAL CARCINOMA CELL LINES

目的 :研究三苯氧胺 (tamoxifen ,TAM)对人乳腺癌Bcap 37和宫颈癌HeLa细胞增殖的影响并探讨其可能的机制。方法 :采用细胞培养、细胞计数、MTT、流式细胞术和激光共聚焦显微镜技术。结果 :TAM ( 10 -6mol/L)使Bcap 37细胞的生长曲线下移 ,使HeLa细胞的生长曲线上移。TAM ( 10 -8～ 10 -6mol/L)剂量依赖性的抑制Bcap 37细胞的增殖 ,促进HeLa细胞的增殖作用。TAM ( 10 -6mol/L)使Bcap 37细胞发生凋亡 ,凋亡率达到 97.5 %,而使HeLa细胞周期由G1期加速向S期转化 ,G1期的DNA含量由对照组的 5 5 .5 %下降到加药组的 32 .8%,S期的DNA含量由对照组的 2 9.0 %上升到加药组的 49.4%。激光共聚焦检测到TAM( 10 -6mol/L)可使Bcap 37细胞和HeLa细胞内的Ca2 +浓度显著升高。结论 :TAM可以通过调节细胞周期各阶段DNA含量和胞内Ca2 +浓度水平 ,从而调节Bcap 37细胞和HeLa细胞的增殖活动 ,提示使用TAM治疗乳腺癌时可能会对子宫颈产生副作用。

Aim: To investigate the effects of tamoxifen on proliferation of human breast cancer Bcap-37 cells and cervical carcinoma HeLa cells and to explore it's possible mechanism. Methods: The techniques of cell culture, growth curves, flow cytometry and laser scanning confocal microscope were used. Results: Tamoxifen(10 -6 mol/L) shifted the growth curve of Bcap-37 cells downward, and shifted the growth curve of HeLa cells upward. Tamoxifen(10 -8 ～10 -6 mol/L) inhibited the proliferation of Bcap-37 cells in a dose-dependent manner, but stimulated the proliferation of HeLa cells in a dose-dependent manner. Bcap-37 cells appeared apoptosis when treated with tamoxifen(10 -6 mol/L), and the same dose stimulated the proliferation of HeLa cells at G 1/S phases. The apoptotic rate of Bcap-37 cells was 97.5%. It blocked G 1 phase of HeLa cells from 55.5% to 32.8%, and increased the S phase from 29.0% to 49.4%. Tamoxifen(10 -6 mol/L) also increased the releasing of calcium in Bcap-37 and HeLa cells. Conclusion: Tamoxifen can significantly influence the proliferation of breast cancer and cervical carcinoma cells possibly by affecting cell cycle and stimulating the releasing of Ca 2+ in the cells.