用条件性逃避反应建立简易的大鼠耳鸣检测方法

ESTABLISHING A SIMPLE METHOD FOR DETECTING TINNITUS OF RATS BY USING BEHAVIORAL RESPONSE OF CONDITIONED ESCAPE

目的 :用较简单的行为实验建立检测动物耳鸣的方法。方法 :按照巴甫洛夫条件反射原理 ,参照Jastreboff的方法 ,建立“中断背景声音 大鼠逃跑”的条件反射 ;不再给电击 ,观察不同组别大鼠条件反射消退过程的行为表现 ,用公认的水杨酸耳鸣造模法来验证本方法的可靠性。结果 :水杨酸组动物停止背景声音时不出现或较少出现逃跑反应 ,因为大鼠耳内仍有声音存在 (耳鸣声 ) ,即条件刺激时有耳鸣的动物出现的逃跑反应次数小于无耳鸣的对照动物。结论 :本实验设计可检测动物是否有耳鸣。

Aim: To create a simple behavioral procedure for detecting tinnitus of rats. Methods: A procedure based on a Pavlovian conditioned suppression paradigm was set referencing Jastreboff's way to detect tinnitus behaviorally in conscious animals. Rats received acquisition training through Pavlovian conditioning to associate the conditioned stimulus (noise offset) with the unavoidable unconditioned stimulus (foot-shock). The manifestation here is escape reaction. An extinction procedure followed, during which the subject was exposed to the conditioned stimulus, but shock is no longer given. The manifestations of the rats in different group during the extinction processes were observed. Tinnitus was induced by salicylate (SA) injection to validate the method. Results: The escaped rate of SA group is lower than that of the control. It indicated that the SA rats escaped seldom when there was a sound off, because the rats might perceive the salicylate-induced auditory sensation as a substitute for the background noise that had acquired a safety value during training. Conclusion: The behavioral procedure could be used to detect tinnitus of rats induced by salicylate.