乙型肝炎病毒相关性肾炎患者肾组织中HBsAg、HBcAg的检测(英文)

Detection of HBsAg and HBcAg in renal tissues from patients with HBV related glomerulonephritis

目的 观察乙型肝炎病毒相关性肾炎 (HBV -GN)患者肾脏组织中HBsAg、HBcAg的表达情况。方法 以 6 3例HBV -GN患者为研究对象 ,另随机选择 2 0例非乙型肝炎病毒相关性肾炎 (NHBV -GN)患者、12例血清HBV标志阳性的其他肾病 (肾结核、肾结石、肾细胞癌、肾萎缩等 )患者作为对照组 ,用免疫组化方法检测肾穿刺活检组织HBsAg、HBcAg的表达。 结果 HBV -GN患者肾脏组织中均可检出HBsAg、HBcAg阳性颗粒 ,阳性率分别为 76 .2 % (4 8/ 6 3) ,4 2 .9% (2 7/ 6 3) ;2 0例NHBV -GN患者肾组织HBsAg、HBcAg均未检出 ,与HBV -GN患者相比有统计学差异 (P <0 .0 5 ) ;12例血清学HBV标志阳性的其它肾病患者肾组织HBsAg、HBcAg阳性率均为 75 % ,与HBV -GN患者相比无统计学差异 (P >0 .0 5 )。血清HBeAg阳性与血清HBeAg阴性的HBV -GN患者相比 ,肾脏组织中HBsAg、HBcAg表达情况经统计学比较 ,无显著性差异 (P >0 .0 5 )。结论 HBV在肾脏中定位及复制 ,不仅可引起肾脏损害 ,并与病毒的保存及乙型肝炎的传播可能有关。

Objective: To investigate the presence of HBsAg and HBcAg in renal tissues from patients with hepatitis B virus related glomerulonephritis(HBV-GN). Methods: Renal tissue ordinary biopsies from 63 patients with HBV-GN and two control groups were prepared for immunocytochemical detection of HBsAg and HBcAg.Results:HBsAg and HBcAg were present with the positive rates of 76.2%, 42.9% respectively in renal tissues from HBV-GN patients, which were higher than those of NHBV-GN patients, but assumed no statistical difference compared with those of other renal patients with positive HBVM. Renal tissue HBsAg and HBcAg positive expression rates showed no statistically significant difference between HBV-GN patients with positive serum HBeAg and HBV-GN patients with negative serum HBeAg. Conclusions: The location and replication of HBV in renal tissue may lead to renal lesions, moreover, it plays a role in the preserve of virus and the transmission of hepatitis B.