急性白血病患者VEGF与多药耐药关系的初步探讨

Relationship between VEGF and MDR in acute leukemia patients

目的 探讨急性白血病 (AL)患者VEGF与MDR的关系。方法 用双抗体夹心酶联免疫法(ELISA)检测 36例化疗后AL患者血浆VEGF含量 ;同时以免疫组化法检测其骨髓单个核细胞Pgp的表达。结果 17例难治 /复发AL患者血浆VEGF含量 (76 .0 1± 4 0 .18) pg/ml较对照者 (33.13± 11.97) pg/ml和完全缓解者 (4 2 .0 7± 17.75 ) pg/ml明显升高 (P <0 .0 1) ;而完全缓解者VEGF含量与正常对照者之间无显著性差异 (P >0 .0 5 ) ;难治 /复发AL患者骨髓单个核细胞Pgp表达阳性细胞率 (2 0 .4 7± 9.96 ) % ,明显高于完全缓解者 (6 .0 0± 4 .17) % ,P <0 .0 1,且VEGF含量与Pgp表达具有一定的相关性 (r =0 .6 6 8,P <0 .0 1)。 结论 AL患者血浆VEGF的检测 ,可作为了解病情、观察疗效、判断预后的指标之一 ;AL患者血浆VEGF升高与Pgp高表达有一定的一致性 ,考虑VEGF可能参与急性白血病MDR的发生。

Objective:To investigate the relationships between VEGF and MDR in acute leukemia(AL).Methods: The plasma VEGF concentration was detected by ELISA and the Pgp expression of nucleated cells in bone marrow was detected by immunohistochemistry in 36 patients with AL.Results: The plasma VEGF concentration in the 17 patients with refractory/relapsed AL(76.01±40.18)pg/ml was significantly higher than that of the control (33. 13±11.97)pg/nl and the complete remiasion group(42.07±17.75) pg/ml,P<0.01, but there were no significant between the remission group and the control.The Pgp positive cell rate of the refractory/relapsed group(20. 47±9. 96)% was hisher auto that of the complete remission group (6. 00± 4. 17)%,P<0. 01, and there were linear correlation between the VEGF coacenuation and the Pgp expression in the refractory/relapsed group (r=0. 668 P<0.01).Conclusions:The plasma VEGF concentration can be one of the indicators for learning clinical features and predicting prognosis of AL patients; VEGFmaytakeapart in the ocanrence of MDR in acute leukemia patiets.