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用选择性指示菌筛选除莠霉素A高产菌株

Screen of herbimycin A high yield strain by selective indicator microorganism
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摘要 以吸水链霉菌NND 5 2菌株为原始菌株 ,利用紫外诱变和LiCl复合处理 ,采用琼脂柱移菌法进行初筛。用清酒酵母作为特异性指示菌 ,经复筛获得一株除莠霉素A高产菌株 ,产量为 980 0 9μg·mL-1 ,比原始菌株提高了 2 2 6倍。 An agar gel transplanting model for mutation pre screening by selective indicator microorganism Saccharomyces sake was built up.Through combined mutation procedure UV+LiCl,take Streptomyces hygroscopicus NND 52 as original strain,a herbimycin A high yield strain was isolated.Its yield increased by 2 26 times,to 980 09 μg·mL -1 .
出处 《南京农业大学学报》 CAS CSCD 北大核心 2004年第1期59-62,共4页 Journal of Nanjing Agricultural University
基金 教育部高校博士点基金资助项目 (B980 0 0 5 )
关键词 选择性指示菌 除莠霉素A 菌株筛选 复合诱变 清酒酵母 抗生素 herbimycin A selective indicator microorganism combined mutation
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