应用6个STR基因座进行奶牛亲子鉴定

Parentage identification of dairy cattle by six STR loci

运用PCR技术用BM 2 1 1 3、BM 1 86 2、BMc 70 1、BM 2 934、TGLA 1 2 2、BM 72 0共 6个STR (短串联重复序列 )基因座对 7头奶牛DNA进行扩增 ,扩增产物分两组混合后经聚丙烯酰胺凝胶电泳和银染检测 ,扩增片段互不干扰。采用已确定母女关系的 2头奶牛血液样本和 5个嫌疑父亲的精液样本 ,对一头母本已知的奶牛在嫌疑父本中找出父本 ,并计算父权概率为 99 993%。结果证明 。

Amplifications of six short tandem repeat(STR)loci including BM 2113, BM 1862, BMc 701, BM 2934, TGLA 122 and BM 720 on DNA of seven dairy cattles were performed by a PCR(polymerase chain reaction)method.The products of amplification were separated in polyacrylamide gel and analyzed with the sliver staining.The paternity probability was calculated by 99 993% on the basis of blood samples from two cows with confirmed paternity and 5 semen samples unknown paternity.The results showed that the PCR with the sliver staining was a validation method for identification parentage in dairy cattle.