摘要
用随机扩增多态DNA (RAPD)技术 ,选用 4个随机引物 ,对 87个大花型香石竹品种总DNA进行随机扩增。 4个引物均得到了稳定的RAPD图谱。扩增出的片段分子量在 5 0 0～ 4 30 0bp之间 ,每个随机引物扩增出的条带数在 8～ 12条之间 ,共扩增出 2 113个条带 ,平均每个引物扩增的DNA条带数为 9 5条。根据DNA谱带计算品种间遗传距离 ,对 87个品种进行了聚类分析 ,分为 10个组群 ,组群间差异较大 。
The technique of random amplified polymorphic DNA(RAPD)was used to amplify the genomic DNA fragments of 87 carnation cultivars by 4 selected arbitrary 10-mer primers,2113 bands were obtained, and the size range was 500-4300 bp.The average number of DNA bands amplified by each primer was 9.5 cluster analysis showed that the extensive genetic variation existed among 87 cultivars of carnation.The 87 cultivars were divided into 10 groups at DNA level.This result has significance for the genetic breeding and identification of cultivars of carnation.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第1期109-111,共3页
Acta Horticulturae Sinica
基金
云南省科技攻关资助项目 ( 95A2 -7)
