醉蝶花离体培养植株再生研究

Studies on Plant Regeneration of Cleome spinosa Jacq.in Vitro

诱导醉蝶花下胚轴和子叶形成愈伤组织 ,以MS +6 BA 0 5mg/L +NAA 0 5mg/L +KT 0 5mg/L培养基中子叶的出愈率最高 ,达到 10 0 % ,下胚轴出愈率最高只有 93%。MS +6 BA 2mg/L +NAA 0 0 5mg/L最适合于芽的诱导 ,诱导率达到 10 0 %。再生苗在MS +NAA 0 1mg/L培养基中生根完成植株再生。带单个侧芽的茎作为外植体接种在MS +6 BA 1mg/L +NAA 0 0 5mg/L培养基中 。

Taking MS as basic media,adopting 6-BA,NAA,KT of different concentration compoundings to induce callus from cotyledons and hypocotyls of Cleome spinosa Jacq.,the result showed that the medium of MS+6-BA 0.5 mg/L+NAA 0.5 mg/L+KT 0.5 mg/L was the best to induce callus from cotyledons with a rate of 100% frequency,and the highest rate of hypocotyl callus inducement was 93%.And the differentiation medium MS+6-BA 2 mg/L+NAA 0.05 mg/L was the best to induce buds and had a 100% plant regeneration.And it was for rooting media,MS+NAA 0.1 mg/L,to produce roots from seedings. Taking the stems with single bud as explants on MS+6-BA 1 mg/L+NAA 0.05 mg/L,it was found that there are lots of buds after a month.