生物活性陶瓷与细胞外基质骨形成对Ca、P、ALP影响

The Effects of Bioactive Ceramics and Extra Cellular Matrices in Relation TO Osteogensis on Ca、P、ALP Value

目的 通过对钙 (Ca)、磷 (P)及碱性磷酸酶 (ALP)评价 ,探讨生物活性陶瓷及细胞外基质与骨细胞相互作用机理 ,为骨替代材料成骨效应提供依据。方法 选用多种材料进行蒸馏水及血浆接触 ,体外成骨细胞培养及体内骨诱导试验。采用原子吸收、钼蓝比色及速率法分析化学性能 ,血浆、细胞冻溶液及组织匀浆中Ca、P、ALP的变化规律。结果 材料组体内、外接触Ca、P、ALP值高于对照组。细胞外基质复合材料组高于相应的非复合材料组 ,TGF -β1加材料高于BMP复合材料组 ,而不同材料有所不同 ,TCP材料高于其它材料。结论 生物活性陶瓷材料均有不同程度的Ca、P离子释放 ,细胞外基质及Ca、P离子可提高成骨细胞活性 ,异位骨形成及ALP活性。

Objective For understanding the effect of new bone formation produced by composite artificial bone, mechanism of the effect between osteoblast and both bioactive ceramics and extracellular matrices (CEM)was investigated by valuation of calcium(Ca),phosphate(P)and alkaline phosphatase (ALP) content.Methods Four kinds of composite ceramics were incubated in distilled water and human serum albumin,cultured with the osteoblost in vitro, and implanted into muscles. The changeful law of Ca, P,ALP centent in different materials and contact way were determined by atomic absorption spectrometry, molybdenum blue colormetry and speed way.Results The contents of Ca? P?ALP were higher in bioactive ceramics(as experimental groups)than control groups in vitro and in vivo test, and were higher in compositive ceramics combined with extracellular matrices than those in nocompositive material groups, and higher in TGF-β 1/ceriamics than in BMP/ceramics. In all experimental groups, the amount of Ca, P,ALP were more in TCP group than other material groups, but there were significant difference between them.Conclusion The bioactive ceramics can release Ca, P,.ion. Extracellular matrices and this ion may increase activation of osteoblast and ALP, and induce ectopic bone formation.