日本血吸虫抗原的表位模拟肽筛选及免疫保护性

Screening of peptide mimics of Schistosoma japonicum male worm and its protecitve immunity

目的 筛选日本血吸虫雄虫抗原的表位模拟肽 ,并探讨其诱导小鼠抗日本血吸虫的免疫保护性。 方法 用日本血吸虫可溶性雄虫抗原的IgG对噬菌体随机 1 2肽库进行亲和筛选。 3轮筛选后 ,经Dot-ELISA检测获得阳性噬菌体克隆 ,并用混合特异性噬菌体克隆免疫小鼠及进行抗日本血吸虫免疫保护性分析。 结果 1 8个特异性噬菌体克隆均显示有抗原性 ,其噬菌体混合克隆免疫诱导小鼠产生了特异性抗体 ,以及 31 72 %的减虫率和 51 54%的减卵率 ,与对照组比较差异有显著性 (P <0 0 0 1 )。 结论 筛选噬菌体肽库获得的日本血吸虫可溶性雄虫抗原的表位模拟肽分子能诱导小鼠产生抗日本血吸虫的保护性免疫。

Objective To select epitope mimics of male worm soluble antigens of Schistosoma japonicum(Sj) and to explore their immune protection in mice. Methods Phage random peptide library of 12 mer was screened with IgG to soluble Sj male worm antigen,Specific positive phage clones selected through three rounds of screenings detected by Dot-ELISA were pooled and applied to mice for vaccination and immunoprotection assessment against Schistosoma japonicum. Results Eighteen randomly picked phage clones all displayed antigenicity.The pooled positive phages detected by ELISA elicited specific antibody in mice ,and 31.72% worm reduction rate as well as 51.54% egg reduction rate in mice,revealing significant difference as compared with that of the controls (P<0.001). Conclusion The epitope mimics of antigenicity and immunogenicity of Sj soluble male worm antigen by phage peptide-display library can elicit partial protection against Schistosoma japonicum.