北京海淀城郊鼠类携带汉坦病毒的相关危险因素研究

Study on risk factors of hantaviruse infection in rodents from Haidian District of Bejjing

目的 了解北京市海淀区城郊宿主动物携带肾综合征出血热病毒状况 ,分析宿主动物带毒相关危险因素。 方法 夜夹法捕鼠 ,计算鼠密度 ,确定鼠种构成。应用RT -PCR扩增M片段部分序列检测宿主带病毒率 ,根据检测结果应用Logistic回归分析通过SPSS( 9 0 )软件分析宿主动物感染的可能危险因素。 结果 共捕获宿主动物 2 3 8只 ,褐家鼠为优势鼠种 ,占 70 5 9%。其次为小家鼠 ,占 2 5 2 1%。RT -PCR检测宿主带病毒率 ,褐家鼠为 14 2 9% ,小家鼠为 0。就褐家鼠而言 ,尽管成鼠阳性率高于幼鼠 ,雄鼠阳性率高于雌鼠 ,但成鼠和幼鼠间、雌鼠和雄鼠间带毒率差异无显著性 (P >0 0 5 ) ,但扩增阳性鼠和扩增阴性鼠体重的差异有非常显著性 (P <0 0 0 1)。多因素分析显示宿主感染危险因素最重要的鼠个体因素尤其是体重。 结论 北京市海淀区城郊汉坦病毒感染的主要宿主动物是褐家鼠 ,采样点的鼠密度与带毒率非正相关 ,但二者可能存在更复杂的关系。

Objective To investigate the condition of hantavirus infection and to determine the risk factors of hantavirus infecnon in rodents captured in Haidian district of Beijing. Methods The captured dead mice were identified and the population density was calculated.RT-PCR was used to amplify the partial M fragments of hantavirus to determine the positive rate. Chi-square test and Logistic regression analysis was used to estimate the risk factors SPSS(9.0) Software.Results A total of 238 animals were captured, among which Battus norvegicus was the dominant specties and the median infection rates with hantavirus were 14.29%in Battus norvegicus and 0 in Mus musculus Linnaeus.Though we found more adults and fewer young and more males and fewer females among the positive Battus norvegicus,there was no significant deference between adult and the young and between males and females. Howerever, the weight of positive mice was significantly heavier than that of negative mice. The major risk factor was the weight of the rodent and the relative factor wihich was associated with positive rates of the host population was the density of host population. Conclusion The major host of hantavirus in Haidian district was Battus norvegicus.Although there was no linear correlation between the density of hosts and prevalence on sample sites, more complex relationships between density and prevalence appeared likely.