强力霉素对心肌间质缺血再灌注损伤影响的研究

Effect of doxycycline on cardial interstitium with ischemic reperfusion injury

目的 :探讨不同浓度的强力霉素对离体鼠心缺血再灌注心肌间质损伤的影响。方法 :Sprague Dawley(SD)大鼠 40只 ,随机分为对照组、缺血再灌注组和强力霉素 1、2、3组 ,每组 8只。分别建立改良的Langendorrf离体心灌注模型 ,测定左室收缩峰压及其压力微分 ,氯胺T法测定心肌胶原含量 ,免疫组化法测定Ⅰ /Ⅲ胶原比例 ,酶谱法检测心肌基质金属蛋白酶 2 (MMP 2 )的表达 ,电镜观察心肌超微结构。结果 :强力霉素对离体心缺血再灌注后心肌间质损伤 ,以及心功能有明显的保护作用 ,且与浓度有关。其保护作用与抑制MMP 2的表达有关。结论 :强力霉素对离体鼠心缺血再灌注后心肌间质损伤有与浓度相关的保护作用。

Objective To assess the effect of different concentration doxycycline on cardial interstitium with ischemic reperfusion (I/R) injury in isolated rat heart. Methods 40 SD rats were randomly divided into five groups: control group (C), ischemic reperfusion group(I/R) and doxycyline 1 group(D1), doxycyline 2 group(D2), doxycyline 3 group(D3) . Each group includes 8 rats. To establish isolated rat heart modified langendorff model retrograde perfusion. The left ventricular peak systolic pressure and its derivate (±dp/dt) were observed. The activity of MMP 2 of myocardium was measured by zymography and callagen amount by the method of chloramines T, and the ratio of Ⅰ/Ⅲ collagen was assessed by immunohistochemical stain. The microstructure of myocardium was observed with electron microscope. Results Doxycycline has protective effect on cardiac interstitial injury with I/R and cardiac function recovery, and there was a concentration dependent increase in the protective effect. Conclusion Doxycycline has concentration dependent protective effect on cardiac interstitial injury with I/R.