聚乙烯颗粒对人单核细胞分泌促炎因子与抗炎因子的影响

The Effect of Polyethylene Particles on Secretion of Pro inflammatory and Anti inflammatory Cytokines by Human Monocytes

【目的】探讨聚乙烯颗粒对人单核细胞分泌促炎因子与抗炎因子的影响。【方法】用Ceridust3615颗粒(聚乙烯颗粒)(平均直径14.27,6.39,1.74,1.01,0.54和0.28μm)分别刺激人单核细胞,颗粒体积与细胞数的比例为100∶1和10∶1。在颗粒刺激细胞24h后,运用ELISA检测培养上清液中的促炎因子(白介素-1β,白介素-6和细胞坏死因子α)及抗炎因子(白介素-10)的表达。【结果】Ceridust3615颗粒可刺激单核细胞分泌白介素-1β(IL-1β)、白介素-6(IL-6)和细胞坏死因子α(TNFα),但未能引起白介素-10(IL-10)的表达。颗粒比例增高可促使单核细胞分泌更多的IL-6和TNFα,却减少IL-1β分泌,对IL-10的分泌则无影响。个体差异使巨噬细胞对同种颗粒的刺激产生不同的反应,其差异可达到30倍。【结论】个体差异是影响人工关节寿命的重要因素,聚乙烯颗粒可刺激单核细胞产生炎因子但对产生抗炎因子(IL-10)的作用不明显,提示促炎因子与抗炎因子的不平衡或许是人工关节松动不断进展的另一重要因素。

To study the effect of polyethylene particles on secretion of pro inflamma tory and anti inflammatory cytokines by human monocytes.Ceridust 3615particles (mean sizes: 14.27, 6.39, 1.74, 1.01, 0.54 and 0.28 μm) were co cultured with the monocytes at particle volume (μm3) to cell number ratios of 10 ∶1 and 100 ∶1. The 24 hour production of osteolytic mediators (interleukin 1β, interleukin 6 and tumor necrosis factor α)and anti inflammatory cytokine (interleukin 10)were assessed by enzyme linked immunoabsorbent assay.Ceridust 3615particles could stimulate monocytes to secret tumor necrosis factor α(TNFα), interleukin 6(IL 6)and interleukin 1β(IL 1β)but no interleukin 10(IL 10). Higher concentrations of particles in the cell culture provoked higher secretions of TNFαand IL 6. For IL 1βthis was vice versa. The particles had no effect on IL 10 secretion. The individual responses of the donors were rather heterogeneous, the maximum difference of cytokine production was 30 fold. [Conclusion]The heterogeneity of human reactivity to polyethylene wear particles appears to be a major factor in determining implant life. The particles could stimulate monocytes to secret pro inflammatory cytokines but had no effect on anti inflammatory cytokines (IL 10) production, it suggests that the imbalance secretion of the pro inflammatory and anti inflammatory cytokines may be the other factor to cause the progress of the artificial joints loose.