上皮性卵巢肿瘤中雌激素受体亚型表达的研究

Expression of the isoforms of estrogen receptor in epithelial ovarian tumors

目的 了解上皮性卵巢癌雌激素受体 (ER)α、β亚型表达在上皮性卵巢癌中的作用。 方法 选取 4 5例上皮性卵巢癌、10例良性卵巢上皮性肿瘤患者的石蜡病理切片 ,用SP免疫组化法测定ERα、ERβ蛋白表达情况 ,并与临床病理特征进行比较。结果 ERα、ERβ阳性染色均位于细胞核内。卵巢上皮性癌中 ,ERα(+) 5 1.1%(2 3 4 5 ) ,与良性肿瘤接近 (5 0 .0 % ) ;ERβ(+) 8.89% (4 4 5 ) ,显著低于良性肿瘤 (5 0 .0 % ) ,ERβ阳性率在卵巢浆液性癌、中低分化及Ⅲ、Ⅳ期癌中显著低于ERα(P <0 .0 1)。结论 ERα、β为核受体。原发性上皮性卵巢癌中ERβ阳性率显著降低 ,ERα ERβ升高可能为卵巢癌发生的一个标志。

Objective To study the role of ERα and ER β in epithelial ovarian tumors. Methods Forty-five cases of epithelial ovarian carcinoma and 10 cases of benign epithelial ovarian tumor admitted in The Fourth People′s Hospital of Wuxi from January 1992 to June 2002 were studied. No chemotherapy or hormone treatment was given before operation. The expression of ER α and ER β was determined by using immunohistochemistry in formalin-fixed paraffin-embedded tissue specimens. The status of ER α and ER β was compared to the clinico-pathological characteristics of ovarian carcinoma. Results ER α and ER β proteins were located inside the nuclei. The positive expression rate of ER α was 51.1% (23/45) for primary epithelial ovarian carcinoma, similar to that for benign ovarian tumors(50.0%). The positive expression rate of ER β was 8.89% (4/45) for primary ovarian carcinoma, significantly lower than that for benign tumors (50.0%). The positive rate of ER β was significantly lower than that of ER α in serous cystadenocarcinoma and poorly differentiated and advanced carcinoma. Conclusion ER α and ER β are nuclear receptors. Downregulation of ER β expression and upregulation of ER α/ER β may be a useful marker for epithelial ovarian carcinogenesis, and ER β is of prognostic significance for epihelial ovarian carcinoma.