红松籽油调节血脂作用研究

Study on Effects of korean Pine Seed Oil on Blood Lipid

目的:探讨红松籽油对老龄大鼠血脂的调节作用。方法:将60只22月龄Wistar大鼠随机分成5组(每组雌雄各半):空白对照组,高脂对照组和三个松籽油处理组。除空白对照组外,其余各组均饲喂高脂饲料,同时,3个松籽油处理组分别饲喂2.0g/kg.d、4.0g/kg.d、8.0g/kg.d剂量的松籽油,连续饲喂8周,每周称量1次各组平均体重。饲喂4周后,取尾血,测定血清中的血脂和血糖(BloodGlucose)。结果与空白对照组比较,各组总胆固醇(TC)均显著高于空白对照组,表明高脂模型建立成功。松籽油处理对大鼠体重增长具有明显的抑制作用(P<0.05)。与高脂对照组比较,松籽油处理组血清BG、TC、高密度脂蛋白胆固醇(HDL-C)无显著差异(P>0.05),而4.0g/kg.d剂量组可以明显降低血清中甘油三酯(TG)的含量(P<0.05),但8.0g/kg.d剂量组低密度脂蛋白胆固醇(LDL-C)含量与高脂对照组比较显著升高(P<0.05)。结论红松籽油具有一定调节血脂作用。

Objective: To probe into the effects of the korean pine seed oil on blood lipid of old rats.Methods:60 old Wistar(22months) rats were randomly divided into 5 groups(half male and half female in each group):normal control group(NC),high fat diet control group(HFD control) and three of the korean pine seed oil treatment groups.Rats were fed with high fat food except NC group rats.At the same time,the oil 2.0g/kg·d,4.0g/kg·d,8.0g/kg·d were given to the three oil treatment groups for 8 weeks,weigh up the average rat weight every week. At the beginning of the 5th week, serum was isolated, measured the index of blood lipid and blood glucose. Results: The content of TC of other groups was more than NC( P <0.05), indicated that model of hyperlipidemia had been set up successfully. It was founded that the content of BG,TC and HDL-C have no significant difference( P >0.05), compared with HFD. But the content of serum TG were significantly lower in 4.0g/kg.d group than in HFD control group( P <0.05). And LDL-C of 8.0g/kg.d group is higher than HFD control group( P <0.05). Conclusion: The korean pine seed oil had the effect of regulating blood lipid metabolism of hyperlipidemia in old rats to some extent.