摘要
利用 8 1个株系组成的Kinmaze(japonica) DV85(indica)重组自交系 (recombinantinbredlines ,RIL)群体 ,采用苗期强迫饲毒的鉴定方法 ,以病情指数作为条纹叶枯病的表型值 ,鉴定亲本及 81个RILs对水稻抗条纹叶枯病毒(ricestripevirus ,RSV)的抗性。利用QTLCartographer软件 ,对水稻条纹叶枯病抗性基因进行检测分析。检测到 3个QTL位点 :qStv1、qStv7、qStv11分别位于第 1、7、11染色体上 ,各QTL的LOD值为 2 44～ 3 83 ,贡献率为 19 8%～3 0 9%。根据抗性基因加性效应的方向 ,在qStv7、qStv11位点上 ,亲本DV85存在抗条纹叶枯病增效基因 ,Kinmaze具有抗条纹叶枯病减效基因 。
Rice stripe disease transmitted by small brown planthopper (Laodelphax striatellus Fall.) is one of the most serious viral diseases in East Asia.The disease is severely epidemic in most rice growing areas where the main cultivars are susceptible or moderately susceptible to rice stripe virus.In this research,a recombinant inbred lines (RILs) population of 81 lines derived from a cross of Kinmaze (japonica)/DV85(indica) by the single seed descent method was used to detect quantitative trait loci (QTL) conferring resistance to rice stripe virus(RSV).The response of the two parents and 81 RILs to RSV were investigated by inoculating seedlings with viruliferous small brown planthopper insects,and scored by the disease rate index.The quantitative trait loci for rice stripe disease resistance were analyzed by QTL Cartographer software.Three QTL controlling RSV resistance were detected on chromosomes 1,7 and 11,respectively.Individual QTL accounted for 19.8%～30.9% of the phenotypic variance in the RILs population.The direction of the additive gene effects at two loci qStv7 and qStv11 coincided with that predicted by phenotypes of the parents.At these two loci,the DV85 alleles increased the resistance to RSV,while at qStv1,the Kinmaze alleles increased the resistance to RSV.
基金
农业部 94 8项目 [2 0 1 0 0 2 (A) ]
跨越计划项目 (编号 :1 990 0 1 1 2 )
江苏省攻关项目 (编号 :BE2 0 0 1 30 5)
江苏省财政项目的资助~~
